Instrument: Illumina Genome Analyzer
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Construction protocol: Total RNA was extracted from the shoots and roots with TRIZOL reagent (Invitrogen, Carlsbad, CA, USA). Total RNAs were purified by electrophoretic separations on a 15% TBE-Urea denaturing PAGE gel. The sRNA regions corresponding to 18- to 30-nucleotide bands were excised and recovered. Subsequently, those sRNAs were 5' and 3' RNA adapter-ligated using T4 RNA ligase. RT-PCR was performed for 18 cycles, and the products were purified and sequenced using an Illumina/Solexa Genome Analyzer at the Beijing Genomics Institute,Shenzhen, China.